Gene Cloning

PCR-TRAP: For positive-selection cloning of PCR products through blunt-end ligation. Allows direct cloning of PCR products without purification.

GenHunter has developed a highly efficient blunt-end PCR-TRAP Cloning System. This system uses a third generation cloning vector that features positive selection for cloning PCR products (see figures). Only recombinant plasmids confer antibiotic resistance, making PCR-TRAP extremely efficient. There is no need for any post-PCR manipulation before cloning, since a significant fraction of PCR products do not contain the 3' overhanging A. The mechanism of this unique cloning system involves the phage Lambda repressor gene, cI, which has been incorporated into the PCR-TRAP Vector. Cloning in the PCR product leads to the inactivation of the repressor gene and thus the expression of the TetR gene. So the PCR-TRAP Cloning System is indeed a cloning TRAP!