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Human IL-24 Antibody, Mouse Monoclonal (Clone 12D3), 25 or 100 uL Ascites [K101-12D3-100 or K101-12D3-025]

$560.00
SKU:
K101-12D3-100
Quantity:
Description
Specifications
Ordering
Documents

Product Description

Interleukin-24 (IL-24, also called Mob-5 or Mda-7) is an important member of the IL-10 family of cytokines discovered by Dr. Peng Liang’s laboratory at Vanderbilt University and it signals through two heterodimeric receptors: IL-20R1/IL-20R2 and IL-22R1/IL-20R2 (References 1-5, see below). His group used comprehensive Differential Display screening and identified over two dozen genes regulated by ras oncogene. They focused on a gene encoding a novel secreted protein designated IL-24, which is expressed by activated T cells as well as other cell types transformed by ras oncogenes. Through a series of biochemical studies including GenHunter's AP-TAG technology, Dr. Liang's group demonstrated that ras oncogenes could not only induce the expression of IL-24, but also its receptor(s) in the transformed cells.

Both IL-24 and IL-20 share the identical heterodimeric receptors. Upon binding to its receptors, IL-24 induces rapid activation of Stat-1 and Stat-3 transcription factors (2) in the JAK-SAT signaling pathway, which appear to play a role in cell survival and proliferation (4). Under physiological conditions, the major sources of IL-24 are the activated monocytes and T helper 2 cells (2-4), whereas the major IL-24 target tissues, based on the receptor expression pattern, are non-hematopoietic in origin and include skin (3-4), lung, and reproductive tissues. IL-24 is part of a complex cytokine network implicated in epidermal functions (3), tissue repairs/wound healing (5), autoimmune diseases such as psoriasis (4), and cancer (6). Thus, the IL-24 signaling pathway may offer a potential therapeutic target for treatment of cancer and other conditions.

GenHunter is now offering 5 monoclonal antibodies against human IL-24, 1 polyclonal antibody against human IL-24, 1 polyclonal antibody against rat IL-24, plus other controls. We also offer 2 human IL-20R2 monoclonal antibodies and 1 fusion protein.

 

IL-24 and IL-20 products available:

Product description Volume Applications

Cat. No.

Click to Order

Human IL-24 Antibody (Clone 2B5)

    Mouse monoclonal (Ascites)

100 µL

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-2B5 Order

Human IL-24 Antibody (Clone 12D3)

    Mouse monoclonal (Ascites)

25 & 100 µL

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-12D3-025
K101-12D3-100
Order

Human IL-24 Antibody (Clone 18F4-C1)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K101-18F4-025
K101-18F4-100
Order

Human IL-24 Antibody (Clone 22H8-G9)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K101-22H8-025
K101-22H8-100
Order

Human IL-24 Antibody (Clone 27D6-F5)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-27D6-025
K101-27D6-100
Order

Human IL-24 Antibody

    Rabbit polyclonal (Antiserum)

100 µL

Western

(at 1:1000-1:2000 dilution)

K102 Order

Rat IL-24 Antibody

    Rabbit polyclonal (Antiserum)

100 µL

Western

(at 1:1000-1:2000 dilution)

K201 Order
IL-24-His (human) Western blot control 100 µL Western K301 Order
IL-24-His (rat) Western blot control 100 µL Western K302 Order

Human IL-24 conditioned media

    Serum-free, hIL-24 at ~1µg/mL

1 & 5 mL

Receptor activation

Working conc.: 50 ng/mL

K401
K402
Order

 

 

 

   

Human IL-20R2 Neutralizing Antibody 

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:1000-1:2000 dilution)

K501N-025
K501N-100
Order

Human IL-20R2 Binding Antibody 

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K501B-025
K501B-100
Order

Recombinant Human IL-20R2-Fc Fusion Protein

25 & 100 µg

 

MP203-025
MP203-100
Order

We also have many other antibody and antigen products (CLICK HERE or names below) including for:
SARS-CoV-2 (COVID-19), Human IL-24, Human IL-20R2, Nplate (Romiplostim), Human VEGF-R, Endo180 (uPARAP, CD280), Tumor Necrosis Factor (TNF) superfamily, SIRPα (Signal-regulatory protein alpha), Rabies virus, TRIMER-TAG, RSV (Respiratory Syncytial Virus)HSV-2 (Herpes Simplex Virus 2)AP (alkaline phosphatase).


References:

  1. Wang, M. and Liang, P.: Interleukin-24 and its receptors. (2005) Immunology. 114:166–170. https://onlinelibrary.wiley.com/doi/10.1111/j.1365-2567.2005.02094.x
  2. Wang, M. et al.: Interleukin-24 (MDA-7/MOB-5) signals through two heterodimeric receptors, IL-22R1/IL-20R2 and IL-20R1/IL-20R2. (2002) J. Biol. Chem. 277:7341-7347. https://www.jbc.org/article/S0021-9258(19)82292-5/pdf
  3. He, M. and Liang, P.: IL-24 transgenic mice: in vivo evidence of overlapping functions for IL-20, IL-22, and IL-24 in the epidermis. (2010) J. Immunol. 1, 184: 1793-1798. https://journals.aai.org/jimmunol/article/184/4/1793/38783/IL-24-Transgenic-Mice-In-Vivo-Evidence-of
  4. Liu, X. et al: A broad blockade of signaling from the IL-20 family of cytokines potently attenuates collagen-induced arthritis. (2016) J Immunol. 197:3029-3037. https://journals.aai.org/jimmunol/article/197/8/3029/105897/A-Broad-Blockade-of-Signaling-from-the-IL-20
  5. Liu, S. et al: A tissue injury sensing and repair pathway distinct from host pathogen defense. (2023) Cell. 186:2127–2143. https://www.cell.com/cell/fulltext/S0092-8674(23)00328-8
  6. Zhang, R. et al: Identification of a novel ligand-receptor pair constitutively activated by ras oncogene. (2000) J. Biol. Chem. 275:24436-24443. https://www.jbc.org/article/S0021-9258(19)62189-7/fulltext

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Antibody Specifications
Antibody Name

Human IL-24 Antibody, Mouse Monoclonal (Clone 12D3)

Clone No. 12D3
Class Mouse Monoclonal (Ascites)
Catalog No / SKU K101-12D3-100 (for 100 µL) or K101-12D3-025 (for 25 µL)
Specificity Human IL-24 (Mob-5 or Mda-7)
Binding / Neutralizing mAb             Binding mAb
Volume 25 or 100 uL
Provided as Liquid
Applications ELISA 
Western Blot at 1:1000 - 1:2000
Concentration Not tested
Purification N/A
Storage conditions Store at -20°C long term, Avoid freeze / thaw cycle

All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.

Documents

AP-TAGbrochure-pdf.jpg

IL-24 and IL-20 products available:

Product description Volume Applications

Cat. No.

Click to Order

Human IL-24 Antibody (Clone 2B5)

    Mouse monoclonal (Ascites)

100 µL

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-2B5 Order

Human IL-24 Antibody (Clone 12D3)

    Mouse monoclonal (Ascites)

25 & 100 µL

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-12D3-025
K101-12D3-100
Order

Human IL-24 Antibody (Clone 18F4-C1)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K101-18F4-025
K101-18F4-100
Order

Human IL-24 Antibody (Clone 22H8-G9)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K101-22H8-025
K101-22H8-100
Order

Human IL-24 Antibody (Clone 27D6-F5)

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:1000-1:2000 dilution)

K101-27D6-025
K101-27D6-100
Order

Human IL-24 Antibody

    Rabbit polyclonal (Antiserum)

100 µL

Western

(at 1:1000-1:2000 dilution)

K102 Order

Rat IL-24 Antibody

    Rabbit polyclonal (Antiserum)

100 µL

Western

(at 1:1000-1:2000 dilution)

K201 Order
IL-24-His (human) Western blot control 100 µL Western K301 Order
IL-24-His (rat) Western blot control 100 µL Western K302 Order

Human IL-24 conditioned media

    Serum-free, hIL-24 at ~1µg/mL

1 & 5 mL

Receptor activation

Working conc.: 50 ng/mL

K401
K402
Order

 

 

 

   

Human IL-20R2 Neutralizing Antibody 

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:1000-1:2000 dilution)

K501N-025
K501N-100
Order

Human IL-20R2 Binding Antibody 

    Mouse monoclonal, purified

25 & 100 µg

Western, ELISA

(at 1:400-1:1000 dilution)

K501B-025
K501B-100
Order

Recombinant Human IL-20R2-Fc Fusion Protein

25 & 100 µg

 

MP203-025
MP203-100
Order
We also have many other antibody and antigen products (CLICK HERE or names below) including for:
SARS-CoV-2 (COVID-19), Human IL-24, Human IL-20R2, Nplate (Romiplostim), Human VEGF-R, Endo180 (uPARAP, CD280), Tumor Necrosis Factor (TNF) superfamily, SIRPα (Signal-regulatory protein alpha), Rabies virus, TRIMER-TAG, RSV (Respiratory Syncytial Virus)HSV-2 (Herpes Simplex Virus 2)AP (alkaline phosphatase).


All products are FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.