For transfection with pAPtag vectors for serum free production

The 293T-S cell line is a clone of our human embryonic kidney (HEK) 293T cell line, but it has already been adapted for use in serum-free (SFM) production. They can be used with APtag-2, pAPtag-4, and pAPtag-5 vectors as well. Just like the standard 293T cells, the fusion proteins can be easily monitored after transfection by alkaline phosphatase assay (See our AP Assay Reagent A, Cat. No. Q501) or by Western blot using AP Antibody. But for long term production of AP fusion proteins, we recommend that a stable cell line be cloned using a co-transfection vector such as our puromycin or hygromycin-resistant plasmids (293T is G418 resistant).