For production of high levels of AP alone

The 293T/pAPtag-4 stable cell line is used to produce high levels of secreted human placental alkaline phosphatase (AP) which can be used as a negative control for a ligand-AP or soluble receptor-AP fusion protein in cell surface binding assays or cell staining. High level production of secreted AP can be achieved with sub-confluent to confluent culture a few days after medium change. The secretion of AP can be monitored easily with the culture medium by AP activity assay using GenHunter AP Assay Reagent A (Cat. No. Q501).