For transfection with pAPtag vectors

293T is a human embryonic kidney (HEK) cell line commonly used for transfection assays. Due to the expression of the large T antigen in the cell, plasmids with SV40 origin of replication (such as pAPtag-2, pAPtag-4, and pAPtag-5) can be transiently transfected and give extremely high levels of expression of AP fusion proteins (e.g. ligand-AP fusion proteins). Thus, we strongly recommend using this cell line for your production of AP fusion proteins with pAPtag vectors. The fusion proteins can be easily monitored 2-3 days after transfection by alkaline phosphatase assay (See our AP Assay Reagent A, Cat. No. Q501) or by Western blot using AP Antibody. But for long term production of AP fusion proteins, we recommend that a stable cell line be cloned by co-transfecting with a puromycin or hygromycin-resistant plasmid (293T is G418 resistant).

5 x 10^6 cell/vial