For transfection of vectors carrying the SV40 region of replication, such as our pAPtag vectors. Perfect for retrovirus production, andenovirus production, gene expression, and protein production.

293T is a human embryonic kidney (HEK) cell line commonly used for transfection assays. Due to the expression of the large T antigen in the cell, plasmids with SV40 origin of replication (such as pAPtag-2, pAPtag-4, and pAPtag-5) can be transiently transfected and give extremely high levels of expression of AP fusion proteins (e.g. ligand-AP fusion proteins). Thus, we strongly recommend using this cell line for your production of AP fusion proteins with pAPtag vectors. The fusion proteins can be easily monitored 2-3 days after transfection by alkaline phosphatase assay (See our AP Assay Reagent A, Cat. No. Q501) or by Western blot using AP Antibody. But for long term production of AP fusion proteins, we recommend that a stable cell line be cloned by co-transfecting with a puromycin or hygromycin-resistant plasmid (293T is G418 resistant).

Scientists use HEK 293 type cells such as these for many applications including amplification and production of andenoviruses, retroviruses, gene expression, and other protein production. Our HEK 293T cells do not contain any adenoviral sequences, nor does it produce any viruses (unless you transfect that sequence).

5 x 10^6 cell/vial

Storage Conditions before use: Long term in liquid N2 or Short term at -80˚C (within 2 weeks)

These cells are for Research Use Only. If you plan to use them for commercial applications, including therapeutic drug production, a separate sublicense is required. Please contact us with questions.

These cells constitutively express the simian virus 40 (SV40) large T antigen, and were selected specifically for its high transfectability. The GenHunter 293T cells are a clonally purified, immortalized cell line that can be passed indefinitely for both transient and stable transfection experiments. GenHunter does not keep track of the passage number as the cells are immortal.

Detailed protocol included.