For immunoblotting of AP fusion proteins
This kit contains the AP Antibody (rabbit polyclonal)
which is specific to human placental secreted AP as
well as two controls for AP Western blots. The antibody
works optimally when used for Western blot
analysis of secreted AP fusion proteins from culture
media. It should be noted that although this antibody
works extremely well for Western blot detection of AP
fusion proteins, AP itself (with a MW of 67 KD) may
not be detected directly from the culture media due to
the amount of albumin which runs at a similar MW.
Therefore, the purified AP from human placenta and a
known soluble receptor AP fusion protein are provided
as positive controls for the antibody. In addition, this
antibody only recognizes the denatured form of AP.
The Monoclonal AP Antibody (Cat. # Q320 - see below) can be
used for applications where recognition of the native
form is required.
| | Cat. No. |
|---|
| AP Western Blot Kit |
Q310 |
|
Individual components for the AP Antibody Kit sold separately
|
| Description | Cat. No. | Volume |
AP Antibody (human placenta) - Polyclonal
From rabbit | Q301 | 100 無 |
Purified AP (Western blot control)
human placenta | Q302 | 100 無 |
| Kit-AP fusion protein control media | Q303 | 200 無 |
For ELISA Assay of Alkaline Phosphatase or IP (Immunoprecipitation)
This Monoclonal AP Antibody (human placental) is purified IgG 2a with a
concentration of approximately 2.3 mg/mL. It is purified by DEAE chromatography
and is in 15mM potassium phosphate buffer, 150mM sodium chloride,
0.1% sodium azide, pH 7.2. This antibody does not recognize denatured AP and
therefore cannot be used for Western Blot directly.
|
| Description | Cat. No. | Volume |
| APAntibody (human placenta) - Monoclonal |
Q320 |
100 痢 |
| APAntibody (human placenta) - Monoclonal |
Q321 |
1 mg |
|
For one-step purification of AP-fusion proteins and proteins interacting with AP-fusion proteins
Applications:
- Concentrating AP fusion protein from the conditioned media
- Purification of AP fusion proteins
- Affinity column purification of protein(s) or molecules interacting with the ligand/receptor-AP fusion protein
- IP Western analysis of the AP fusion proteins (see Polyclonal AP Antibody)
Technical parameters:
Coupling condition: 1 mg of pure IgG/mL of beads
AP binding capacity: up to 200 Units/mL of beads
Specificity of the antibody: Native human placental AP
Interference of AP activity: The antibody binding does not interfere with the AP activity
Product Form:
The Monoclonal AP antibody-sepharose beads are supplied in a 50% suspension in 0.1 M NaHCO3, 0.5 M NaCl, pH 8.4.
|
| Description | Cat. No. | Volume |
| AP Antibody-Sepharose Beads |
Q330 |
100 無 |
| AP Antibody-Sepharose Beads |
Q331 |
500 無 |
| AP Antibody-Sepharose Beads |
Q332 |
1 mL |
|
For eluting AP fusion protein from the Monoclonal AP Antibody-Sepharose Beads
This specially formulated elution buffer can break the extremely
tight interaction between the antibody-antigen, allowing up to
60% recovery of the bound antigen.
|
| Description | Cat. No. | Volume |
| Antigen Elution Solution |
Q340 |
10 mL |
| Antigen Elution Solution |
Q341 |
50 mL |
|
Want to save money on Antibodies and get a PerfectWestern?
For information on our PerfectWestern Containers, see the PerfectWestern Containers page.
These containers come in 12 different sizes to fit different size membranes. This allows you to save money on expensive antibodies because significantly less volume can be used.
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