| | For AP Activity Assay
The AP assay reagent A is formulated specifically for measuring the enzymatic activity
of the alkaline phosphatase (AP). The secretion of human placenta AP or a secreted ligand(receptor)-AP
fusion protein can be easily monitored by this assay using the culture media in which the transfected
cells are grown. The dephosphorylation of the p-Nitrophenyl Phosphate by AP leads to the generation
of yellow color which serves as both qualitative (by eye) and quantitative (Absorbance 405 nm)
measurement of AP activity.
Detailed protocol included.
References:
- Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.
| | Cat. No. |
|---|
| AP Assay Reagent A | Q501 |
|
Detailed protocol for cloning included.
Shipped on dry ice via overnight delivery. |
|
For Cell Staining
The AP Assay Reagent S is formulated specifically for cell staining or Western blotting
analysis of enzymatic activity of the alkaline phosphatase (AP). The AP substrate BCIP upon dephosphorylation
forms an insoluble blue precipitate, thus it can be used for tissue or cell staining for the presence of
receptors to which the ligand-AP fusion proteins bind. This assay kit can also be used during expression
cloning of a receptor (Cheng and Flanagan, 1994, Cell 79:157-168).
Detailed protocol included.
References:
- Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.
| | Cat. No. |
|---|
| AP Assay Reagent S | Q502 |
|
|
| | Cat. No. |
|---|
| HBHA Wash Buffer | Q503S | 100 mL |
| HBHA Wash Buffer | Q503L | 500 mL |
For Receptor Binding Assay
HBHA wash buffer consists of Hank's balanced salt solution with 0.5 mg/mL BSA and 20 mM
HEPES, pH 7.0. This buffer has been used extensively for ligand-receptor binding assays.
Detailed protocol included.
References:
- Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.
| | Cat. No. |
|---|
| Cell Lysis Buffer | Q504 | 100 mL |
For Cell Lysis in Receptor Binding Assay
The cell lysis buffer is used in ligand-receptor binding assay. This buffer allows rapid lysis
of the cells and removal of cell nuclei before bound AP activity is measured.
Detailed protocol included.
Refernces:
- Flanagan, J. G. and Leder, P. (1990). The kit Ligand: A cell surface molecule altered in steel mutant fibroblasts. Cell 63, 185-194.
|
As the world-wide exclusive licensee for the
AP-TAG technology reagent business, GenHunter Corporation is pleased to announce its effort to build up a collection of AP-bodies (Ligand or soluble
receptor-AP fusion proteins) made with AP-TAG technology and make them available to the biomedical research community. If you have already made and/or
published an AP fusion construct to your ligand or soluble receptor gene of interest, please consider depositing your vector constructs and the stable
production cell lines with GenHunter. In return, GenHunter will provide you or your lab with 6% royalty on sales of any products associated with your
deposition. Please contact GenHunter for details regarding such depositions (800-311-8260, Fax: 615-832-9461, or email: info@genhunter.com).
Please be reminded that, according to our AP-TAG licensing agreement with Harvard, even if you obtained the APtag vectors from Harvard prior to
March 1, 1999, when our exclusive sublicensing agreement went into effect, you do not have the right to distribute the APtag vectors or their derivatives
such as a ligand-AP fusion construct or cell line harboring such a construct to others without written permission from GenHunter Corporation.
Please consider depositing your AP-body with GenHunter, which saves you not only from legal issues regarding AP-TAG technology, but also
hassles in dealing with end-user requests.
AP-bodies Now Available
All AP-bodies are supplied as culture medium from the corresponding production cell line. Minimum amount of AP activity will be between 0.6 to 1 units/mL,
unless otherwise specified. All AP-bodies are filter-sterilized and buffered with 20 mM HEPES, pH 7.5. Recommended short term storage
(less than 1 month) is at 4°C and longer storage should be at -20°C.
| AP-body | Receptor/Ligand Recognized | Cat. No | Volume | Price |
| AP control | Negative control | Q701 | 50 mL |
| Kit-AP | Stem Cell Factor | Q702 | 50 mL |
| ELF2-AP | Elk, Cek5, Cek10 | Q703 | vial |
| Sema3A-AP | Neuropilin | Q704 | vial |
| mP84-AP | IAP/CD47-AP | Q705 | vial |
| IAP/CD47-AP | mP84-AP | Q706 | vial |
| |
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