Dr. Fukata's ADAM22 binding |
Dr. Hui Sun, UCLA |
Here we highlight 2 recent publications from Science magazine that used our AP-TAG technology: one from a Japanese group studying epilepsy and the other from a group at UCLA studying Vitamin A absorption.
It is known that most inherited forms of epilepsy result from mutations in ion channels which cause abnormally synchronized synaptic transmission in the brain. However, one form of epilepsy, autosomal dominant partial epilepsy with auditory features, is characterized by mutations in a secreted neuronal protein, LGI1. Using both immunoprecipitation and GenHunter's AP-TAG technology, a group of Japanese scientists led by Dr. Masaki Fukata from the National Institute of Longevity discovered that ADAM22, a transmembrane protein that when mutated itself causes seizures, is the receptor for LGI1. LGI1 when bound to ADAM22 enhances AMPA receptor-mediated synaptic transmission in hippocampal slices. These studies opened new avenues for understanding human epilepsy.
To read the Epilepsy paper, Click here.
Complete reference:
Fukata Y., Adesnik H., Iwanaga T., Bredt D.S., Nicoll R.A., and Fukata M.: Epilepsy-related ligand/receptor complex LGI1 and ADAM22 regulate synaptic transmission. Science. 2006 Sep 22; 313:1792-1795.
Vitamin A has diverse biological functions during development. It is transported in the bloodstream in complex with retinol binding protein (RBP), however the molecular mechanism by which vitamin A is absorbed into the cell from the vitamin A-RBP complex is obscure. Using GenHunter's AP-TAG technology, scientists from UCLA including Hui Sun discovered a receptor for RBP in bovine retinal pigment epithelium cells. The receptor STRA6 is a multi-transmembrane domain protein with high affinity to RBP and has robust vitamin A uptake activity from the vitamin A-RBP complex. This breakthrough study revealed that the RBP receptor represents a major physiological mediator of cellular vitamin A uptake.
To read the Vitamin A paper, Click here.
Complete reference:
Kawaguchi R., Yu J., Honda J., Hu J., Whitelegge J., Ping P., Wiita P., Bok D., and Sun H.: A membrane receptor for retinol binding protein mediates cellular uptake of vitamin A. Science. 2007 Feb 9; 315:820-825.
To learn more about our AP-TAG technology please click here.
GenHunter has also compiled a selected list of over 200 publications using our AP-TAG technology, which is growing in usage each year. That list can be found here.
Please feel free to contact us with any questions or comments.
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